To date, this disease has only been reported in farmed Atlantic salmon in Norway, and from a single outbreak in farmed Atlantic salmon in Scotland. Heart and skeletal muscle inflammation (HSMI) is an infectious disease which was first diagnosed by histology in 1999 in farmed Atlantic salmon Salmo salar from Norway. This commercial affiliation does not alter the authors’ adherence to PLOS ONE policies on sharing data and materials. The specific roles of this author are articulated in the ‘author contributions’ section.Ĭompeting interests: This study was funded by Marine Harvest Canada. The funder provided support in the form of salaries for authors (DBM), but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The work is made available under the Creative Commons CC0 public domain dedicationĭata Availability: All relevant data are within the paper and its Supporting Information files.įunding: This study was funded by Marine Harvest Canada.
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This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. Received: JAccepted: OctoPublished: November 4, 2015 PLoS ONE 10(11):Įditor: Vincent Laudet, Ecole normale superieure de Lyon, FRANCE (2015) Piscine Reovirus: Genomic and Molecular Phylogenetic Analysis from Farmed and Wild Salmonids Collected on the Canada/US Pacific Coast. In summary, PRV was genetically homogenous over a large geographic distance (Alaska to Washington State), and the sequence types were relatively stable over a 13 year period.Ĭitation: Siah A, Morrison DB, Fringuelli E, Savage P, Richmond Z, Johns R, et al. Individual analysis of each of the 10 partial segments indicated that the Canadian and US PRV sequence types clustered separately from available whole genome sequences of some Norwegian and Chilean sequences for all segments except the segment S4. Representative PRV positive samples from farmed and wild fish in British Columbia and Washington State were subjected to genome sequencing using next generation sequencing methods.
Comparative analysis of the phylogenetic tree indicated that Canada/US Pacific Northwest sequences formed a subgroup with some Norwegian sequence types (group II), distinct from other Norwegian and Chilean sequences (groups I, III and IV).
In addition, PRV positive samples from fish derived from Alaska, British Columbia and Washington State share identical sequence types. Identical sequence types were found in fish sampled in 2001, 20. Sequence analysis revealed no identifiable geographical or temporal variation among the sequence types. Tissues testing positive for PRV were partially sequenced for segment S1, producing 71 sequences that grouped into 10 unique sequence types. This study examined the phylogenetic relationships among different PRV sequence types present in samples from salmonids in Western Canada and the US, including Alaska (US), British Columbia (Canada) and Washington State (US). Piscine reovirus (PRV) is a double stranded non-enveloped RNA virus detected in farmed and wild salmonids.
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